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Laboratory Technician

  • Location: Bethesda, MoCo, Maryland
  • Salary: 25.53-30.79 per hour
  • Job Type:Contract

Posted about 1 month ago

  • Sector: Government
  • Start Date: 2021-12-22
  • Job Ref: 21-28122
Laboratory Technician
Overall Position Summary and Objectives Laboratory Technician for NIH/NINDS/Viral Immunology and Intravital Imaging Section (VIIIS) to perform a variety of laboratory techniques and procedures.
Minimum Education
Bachelor's

Certifications & Licenses
  • Bachelor's degree in Biology, Life Sciences or a related discipline
  • Minimum of two (2) years of related experience
Skills
  1. Animal Handling: Non-human primates
  2. Excellent analytical, organizational and time management skills
  3. Strong communications skills, both oral and written
1, 2, 3, 4, 5 represents priority rankings, where 1 is highest priority and 5 is lowest priority of those ranked
 
Software
  • NIH POTS
  • Outlook
Field of Study
  • Neuroscience
  •  
DELIVERABLES
  • Clean Equipment - Daily
  • Work products and documents related to performing laboratory techniques and procedures, including handling infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and bright field microscopy. - Daily
  • Work products and documents related to performing digital flow cytometry, ELISAs, DNA/RNA isolations, PCR, RT-PCR, quantitative PCR and Western blots. - Daily
  • Work products and documents related to working with staff on the maintenance of transgenic mouse colony, cell lines, logs and database. - Daily
  • Work products and documents related to inventory supplies and equipment; prepare purchase requests. - Daily
STATEMENT OF WORK DETAILS
 
Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
  •  
  • Perform a variety of laboratory techniques and procedures, including handling infectious agents, performing immune cell isolations, immunohistochemistry, tissue sectioning, fluorescence and brightfield microscopy
  • Perform digital flow cytometry, ELISAs, DNARNA isolations, PCR, RT-PCR, quantitative PCR and Western blots
  • Work with staff on the maintenance and screening of transgenic mouse colony
  • Work with staff on the maintenance of laboratory cell lines
  • Work with staff on the maintenance of laboratory logs and databases as well as prepare inventory of laboratory supplies and equipment, make recommendations, and prepare purchase requests
 
Operates laboratory machines and instruments; and prepares specimens and equipment for use.
  • Prepare materials and equipment for experiments
  • Process and organize multiple samples simultaneously
  • Work with a variety of routine and specialized laboratory instruments and equipment in the laboratory
  • Ensure that all specimens and equipment necessary for experiments are available and in good order
  • Prepare specimens and equipment for use in the laboratory
Performs sample management and storage.
  • Store new samples, update database, retrieve samples with proper authorization
  • Perform processing, description and analysis of samples
  • Work with staff to implement storage and distribution of biological samples for future scientific research
Analyzes, documents and reports on all procedures performed.
  • Convey and discuss research results with team lab members
  • Collect and enter research data into laboratory notebooks and databases
  • Collect data and prepare for analysis and interpretation
Maintains inventory of laboratory supplies.
  • Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory
Cleans and sterilizes instruments, glassware and equipment.
  • Ensure that adequate clean inventory is available for immediate usage
Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
  • Perform fundamental procedures required for research projects
  • Perform a variety of laboratory techniques and procedures
  • Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence immunohistochemistry
  • Perform experiments on samples according to protocols
  • Perform routine experiments and tests; follow established procedures and protocols
Maintains inventory of laboratory supplies and initiates purchase orders.
  • Work with staff to maintain stocked supplies and records of working reagents, including fixatives, buffers, solvents and staining
  • Ensure that reagents are made correctly and that expired reagents or defective products are replaced to ensure validity of results
  • Order and maintain laboratory supplies, reagents and equipment
Performs routine equipment maintenance.
  • Assist in troubleshooting and calibrating various laboratory equipment and instruments
Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.
  • Perform a variety of laboratory techniques and procedures
  • Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence immunohistochemistry
  • Perform routine experiments and tests; follow established procedures and protocols
Analyzes, documents and reports on all procedures performed and produces detailed reports for use by senior staff.
  • Record details of experimental procedures, results and protocols
  • Collect, analyze and interpret data; prepare summaries and reports based on results and findings
Performs advanced equipment maintenance.
  • Assist in troubleshooting and calibrating various laboratory equipment and instruments
functional responsibility
Trains staff in performing routine laboratory tests and procedures.
  • Provide technical help and expertise to lab members
  • Work with staff to monitor and ensure that appropriate laboratory safety courses and certifications for staff are up to date
Provides insights and expertise and recommends new strategies and approaches.
  • Engage in problem solving as required to solve technical difficulties and to ensure steady progress, with a goal of providing high quality experimental data suitable for publication
  • Evaluate new types of experimental approaches analyses and recommend changes to protocols
  • Determine when modifications and adjustments are necessary for experiments
  • Develop and assist in new assays for application based on requests from investigators
  • Troubleshoot issues with technical procedures; utilize trial and error methods customize protocols to the biological systems utilized in the laboratory